Long Noncoding RNA SCIRT Promotes HUVEC Angiogenesis via Stabilizing VEGFA mRNA Induced by Hypoxia

Figure 2

SCIRT is identified as a positive regulator of angiogenic effect under HR condition. (a) SCIRT exhibited the highest correlation score within top 20 upregulated lncRNAs in the HR vs. NC group. (b) qPCR validation of increased SCIRT expression in the HR vs. NC group. (c) Relative expression levels of SCIRT in HUVECs after transfection of SCIRT siRNAs (siRNA1 and siRNA2) and control siRNAs (siCtrl). (d) Inhibition of SCIRT downregulates the protein levels of VEGFA. HUVECs were transfected with SCIRT siRNA, the expression of VEGFA was analyzed by western blot. (e) Tube formation, migration and invasion assay demonstrated that angiogenesis ability was decreased by the knockdown of SCIRT in HUVECs (scale bar, 100 μm). Quantitative analysis of (f) tube formation assay and (g) wound healing assay, respectively. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)