Amelioration of Radiation-Induced Cell Death in Neuro2a Cells by Neutralizing Oxidative Stress and Reducing Mitochondrial Dysfunction Using N-Acetyl-L-Tryptophan

Figure 1

Determination of radiosensitivity of Neuro2a cells and the radioprotective activities of L-NAT using MTT assays. (a) The toxicity effect of L-NAT in Neuro2a cells was measured at different concentrations of L-NAT (1.95-1000 μg/ml) at different times (24-72 h). (b) Radiation-induced sensitization in Neuro2a cells was assessed using different radiation doses (4-70 Gy) at variable time intervals. (c) The radioprotective efficacy of L-NAT against gamma radiation (LD₅₀; 20 Gy) was estimated in Neuro2a cells. Statistical significance was calculated using one-way ANOVA with Turkey, and the p-value was presented as p <0.05; p <0.01; p <0.001 with respect to irradiated control. ^#p <0.001 with respect to control.

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