Amelioration of Radiation-Induced Cell Death in Neuro2a Cells by Neutralizing Oxidative Stress and Reducing Mitochondrial Dysfunction Using N-Acetyl-L-Tryptophan

Figure 11

Analysis of apoptosis regulating protein expression in L-NAT pretreated and irradiated Neuro2a cells. Quantitative analysis of protein expression of Caspase-3 (a), γ-H2aX (b), p53 (c), ERK-1/2 (d) and p-ERK-1/2 (e) was performed in Neuro2a cells after L-NAT and gamma radiation treatment. (f) Western Blot representative images of Caspase-3, γ-H2ax, p53, ERK-1/2, and p-ERK-1/2 were provided. (g) Ponceau staining was also performed to detect the total protein present in the samples. Statistical significance was calculated using Student’s unpaired t-test and p-value presented as p <0.05; p <0.01; p <0.001 with respect to irradiated control, ^#p <0.001with respect to control. NOTE: Ponceau staining of total proteins was used as a positive control in this study because standard housekeeping proteins (HKPs), i.e., GAPDH, tubulin, and actin, were found modulated by irradiation in the Neuro2a cells (Data not shown).

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