Amelioration of Radiation-Induced Cell Death in Neuro2a Cells by Neutralizing Oxidative Stress and Reducing Mitochondrial Dysfunction Using N-Acetyl-L-Tryptophan

Figure 2

Determination of radioprotective activities of L-NAT in terms of reproductive survival against ionizing radiation using SRB assays. (a) Effect of radiation on cellular reproducibility in Neuro2a cells was analyzed at different radiation doses (4-20 Gy) at 72 h. (b) L-NAT pretreatment was assessed for cellular reproducibility in Neuro2a cells against gamma radiation (LD₅₀; 20 Gy) with different concentrations of L-NAT (0.001-10 μg/ml) at 72 h. Error bars are mean ± SEM of triplicate measurement (n =3). Statistical significance was calculated using one-way ANOVA with Turkey and the p-value presented as p <0.05; p <0.01; p <0.001 with respect to irradiated control. ^#p <0.001with respect to control.

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