Amelioration of Radiation-Induced Cell Death in Neuro2a Cells by Neutralizing Oxidative Stress and Reducing Mitochondrial Dysfunction Using N-Acetyl-L-Tryptophan

Figure 6

Gamma radiation mediated neurite outgrowth induction in Neuro2a cells and its inhibition by L-NAT pretreatment to irradiated cells. (a) Phase contrast micrographs (10x magnification; Nikon Ti, Japan) were demonstrated radiation dose (4-24 Gy) dependent neurite outgrowth in Neuro2a cells. (b) The effect of L-NAT pretreatment (0.01-0.08 μg/ml) on neurite outgrowth development in non-irradiated and (c) irradiated Neuro2a cells was analyzed post 72 h. Each scale bar represents 100 μm. (d) Quantitative analysis of radiation doses (4-24 Gy) dependent neurite outgrowth of Neuro2a cells. Arrow marks represent neurite outgrowth and swollen/shrunken cells. (e) Quantitative analysis of L-NAT pretreatment on radiation-induced neurite outgrowth of Neuro2a cells. Error bars are mean ± SEM of triplicate measurement (n =3). Statistical significance was determined and p-value presented as ^#p <0.05; ^##p <0.01; ^###p <0.001 with respect to control. p <0.05; p <0.01; p <0.001 with respect to irradiated control.

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