Amelioration of Radiation-Induced Cell Death in Neuro2a Cells by Neutralizing Oxidative Stress and Reducing Mitochondrial Dysfunction Using N-Acetyl-L-Tryptophan

Figure 9

Role of L-NAT pretreatment on calcium homeostasis regulation in irradiated Neuro2a cells. (a) L-NAT pretreatment (0.04 μg/ml) regulates cytosolic calcium levels in irradiated Neuro2a cells at time points (4-48 h). (b) L-NAT pretreatment to irradiated cells indicated reduced mitochondrial calcium levels compared to irradiated cells. (c) Apart from calcium homeostasis, L-NAT pretreatment also regulates endoplasmic reticulum stress as observed using ER-specific ER tracker Black/White DPx probe (5 μM) in Neuro2a cells. Error bars are mean ± SEM of triplicate measurement (n =3). Statistical significance was determined and p-value presented as p <0.05; p <0.01; p <0.001 with respect to irradiated control,^#p <0.001with respect to control.

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