Dimethyl Sulfoxide Attenuates Radiation-Induced Testicular Injury through Facilitating DNA Double-Strand Break Repair

Figure 3

DMSO radioprotects germ cells in vivo and in vitro. (a) Representative images of the Ki67-stained testis and (b) quantitative analysis are shown at the indicated days post-IR. . (c) Cell survival was detected by CCK-8 after irradiation at various dosages (0-14 Gy). Data from all irradiated samples were normalized to unirradiated samples, and percentages of viable cells were plotted. (d) Representative images of cell plates showing colonies derived from DMSO- or vehicle-treated GC-2 cells either left unirradiated or irradiated with 10 Gy and 12 Gy. (e) Quantitative analysis showing the percentages of surviving colonies. Error bars indicate (). , , , and . Student’s -tests were used to determine statistical significance in (b), (c), and (e).

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