Dimethyl Sulfoxide Attenuates Radiation-Induced Testicular Injury through Facilitating DNA Double-Strand Break Repair

Figure 5

DMSO suppresses radiation-induced germ cell apoptosis in vivo and in vitro. (a) Representative images of the TUNEL-stained testis 12 h post-IR, . (b) Quantification of TUNEL-positive cells. (c) Representative images of the cleaved caspase-3-stained testis 12 h post-IR, . (d) Quantification of cleaved caspase-3-positive cells. (e) Representative flow cytometric analysis of apoptosis in GC-2 cells 24 h post-IR. (f) Percentages of Annexin V-positive GC-2 cells. Error bars indicate (). , . Student’s -tests were used to determine statistical significance in (b), (d), and (f).

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