Dimethyl Sulfoxide Attenuates Radiation-Induced Testicular Injury through Facilitating DNA Double-Strand Break Repair

Figure 7

DMSO facilitates DNA damage repair with a bias toward homologous recombination. (a) The testis was harvested 12 h after irradiation and stained with γ-H2AX. Images were taken with a confocal microscope; γ-H2AX foci are shown (b). (c) The testis was harvested at 0 h, 6 h, 12 h, and 24 h post-IR. WB analysis was performed to examine the protein levels of γ-H2AX, RAD51, BRCA1, and p-BRCA1. Vinculin was used as a loading control. (d) GC-2 cells were harvested at 0 h, 0.5 h, 6 h, 12 h, 24 h, and 48 h after irradiation and stained with γ-H2AX. γ-H2AX foci are shown in green, and DAPI is colored in blue. Images were taken with a confocal microscope. (e) Quantitative analysis of γ-H2AX foci in GC-2 cells. (f) GC-2 cells were harvested at 0 h, 3 h, 6 h, 12 h, 24 h, and 48 h after irradiation, and WB analysis was performed to examine the protein levels of γ-H2AX, RAD51, BRCA1, and p-BRCA1. Vinculin was used as a loading control. Error bars indicate (). , , , and . Student’s -tests were used to determine statistical significance in (b) and (e).

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