Senotherapy Protects against Cisplatin-Induced Ovarian Injury by Removing Senescent Cells and Alleviating DNA Damage

Figure 1

Metformin and DQ independently reduced senescent granulosa cells and SASP caused by cisplatin in vitro. (a) Flowchart of primary GCs in vitro experiment. (b, c) Light microscopic images of SA-β-gal-stained GCs after cisplatin administration and quantitative analysis of SA-β-gal–positive GCs ( =3). Scar bars are marked on respective images. (d–f) Cytokines accumulated in cell culture medium of the NC and Cis groups. (g–l) The protein levels of senescence-associated markers and SASP were analyzed by western blotting ( =3). Results are presented using mean ± standard deviation values. , , .

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