Senotherapy Protects against Cisplatin-Induced Ovarian Injury by Removing Senescent Cells and Alleviating DNA Damage

Figure 3

Metformin and DQ alone or with each other improved healthy ovarian reserve and function in cisplatin-treated mice. (a, h) Representative images of Sirius red stained ovaries and quantitative analysis of Sirius red positive area. Scar bars were marked on respective images ( =3). (b, c) HE staining of ovarian sections and quantitative counting of follicles at different levels ( =5). Black arrow: primordial follicle; red arrow: primary follicle, secondary follicle and antral follicle; yellow arrow: atretic follicle. (d) Regular and irregular estrous cycle percentage of different medication groups ( =6). (e) Mice ovarian index in different medication groups ( =10). (f, g) Serum AMH ( =8) and E₂ ( =7) of following the administration of different drugs. Results are presented using mean ± standard deviation values. , , .

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