Lycopene Ameliorates Hypoxic Pulmonary Hypertension via Suppression of Oxidative Stress

Figure 7

Lycopene alleviated hypoxia-induced oxidative stress. (a, b) The levels of SOD in lungs and serum of rats. (c, d) The levels of MDA in lungs and serum of rats. (e) HIF-1α and NOX4 expressions in the lungs of rats were detected by western blot. β-Actin was used as a loading control. (f, g) The quantification of HIF-1α and NOX4 protein levels in lungs of rats. (h) Immunofluorescence staining for HIF-1α (in red) and NOX4 (in green) in lung tissues of rats. Cell nuclei are counterstained with DAPI (in blue). Scale bars: 50 μm. (i) HIF-1α and NOX4 expressions in PASMCs were detected by western blot. β-Actin was used as a loading control. (j, k) The quantification of HIF-1α and NOX4 protein levels in PASMCs. (l, m) The intracellular ROS was detected in PASMCs by the DCFH-DA assay kit. Values are (-5). , , and vs. the normoxia group; ^#, ^##, and ^### vs. the hypoxia group.

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