[Retracted] Protective Effects of Amauroderma rugosum on Doxorubicin-Induced Cardiotoxicity through Suppressing Oxidative Stress, Mitochondrial Dysfunction, Apoptosis, and Activating Akt/mTOR and Nrf2/HO-1 Signaling Pathways

Figure 8

Histological analysis of heart tissues in the mouse model. Heart tissue samples () were fixed with 4% () formaldehyde and cut into 6 μm sections. (a) Sections were stained with hematoxylin and eosin to evaluate tissue architecture. Black arrows indicate lesions in the heart tissues. Sections were also stained with (b) DHE and (c) TUNEL to investigate ROS accumulation and apoptosis in heart cells, respectively. Sections were labeled with (d) anti-Nrf2 and (e) anti-HO-1 antibodies to evaluate oxidative stress in heart tissues. Red and green signals represent positive signals. Blue signals indicate cell nuclei inside the heart tissues. Scale bar: 100 μm. Quantitative analysis of (f) DHE, (g) TUNEL, (h) Nrf2, and (i) HO-1 signals in heart tissues. Data are presented as the percentage of control (saline) group ( of three independent experiments). indicates a statistically significant difference.