lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients

Figure 1

lncRNA IGF2-AS affected the activity of EPCs in sepsis patients. (a) Cell surface markers CD133, CD34, CD31, and CD45 were detected by flow cytometry. (b) IF was performed to identify EPCs in the Healthy and Sepsis groups. (c) qRT-PCR was applied to detect lncRNA IGF2-AS expression in EPCs of sepsis patients. (d) EdU was used to detect the proliferation of EPCs. (e) Pyroptosis of EPCs was detected by TEM. (f, g) qRT-PCR and WB were utilized to measure pyroptosis-related factors ASC, Caspase-1, NLRP3, and IL-18 expressions. vs. Healthy or si-NC.

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