lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients

Figure 3

Knocking down lncRNA IGF2-AS down-regulated dNTP metabolization-related enzymes. (a, b) After knocking down lncRNA IGF2-AS, the expressions of RRM2, TK1, and TYMS were measured by qRT-PCR and WB. (c, d) After knocking down HMGA1, qRT-PCR, and WB was applied to detect expression of RRM2, TK1, and TYMS. (e) Proliferation of EPCs was detected by CCK-8 after knocking down HMGA1. (f) After knocking down HMGA1, IF was used to detect the expression of Caspase-1. vs. si-NC.

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