Research Article
lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients
Figure 5
Overexpression of lncRNA IGF2-AS reduced the level of dNTP, while dNTPs could save the phenotype of lncRNA IGF2-AS. (a) qRT-PCR verification of lncRNA IGF2-AS overexpression efficiency. (b) dNTP content was measured by GC-TOFMS. (c) Proliferation of EPCs was detected by CCK-8. (d) qRT-PCR and WB were used to detect the expression of pyroptosis-related factors ASC, Caspase-1, NLRP3, and IL-18. (e) IF tested the expression of Caspase-1. vs. oe-NC, # vs. oe-lncRNA IGF2-AS.
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