[Retracted] Chrysophanol Ameliorates Hemin-Induced Oxidative Stress and Endoplasmic Reticulum Stress by Regulating MicroRNA-320-5p/Wnt3a Pathway in HT22 Cells

Figure 5

CHR inhibited the cell apoptosis, ER stress, and oxidative stress by upregulating Wnt3a in hemin-induced HT22 cell injury models. Sh-NC: negative control shRNA; sh-Wnt3a: Wnt3a shRNA. (a) Protein expression levels of Wnt3a, T-β-catenin, and N-β-catenin were analyzed in hemin-induced HT22 cells (20 μM) with or without CHR (10 μM; treatment time: 24 h) or sh-Wnt3a (treatment time: 24 h) following the western blot technique. , . . (b) Apoptosis rate in hemin-induced HT22 cells (20 μM) with or without CHR (10 μM; treatment time: 24 h) or sh-Wnt3a (treatment time: 24 h) was studied by conducting the TUNEL staining assays. 1. . (c) The protein expression levels of ER stress-related genes (p-eIF2α, CHOP, GRP78, and cleaved caspase-12) were analyzed in hemin-induced HT22 cells with or without CHR (10 μM; treatment time: 24 h) or sh-Wnt3a (treatment time: 24 h) using the western blot technique. , , and . . (d) The change of oxidative stress-related indexes (MDA, SOD, GPx, and ROS) were analyzed in hemin-induced HT22 cells with or without CHR (10 μM; treatment time: 24 h) or sh-Wnt3a (treatment time: 24 h). , , and . .

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