Inhibition of MicroRNA-182/183 Cluster Ameliorates Schizophrenia by Activating the Axon Guidance Pathway and Upregulating DCC

Figure 4

miR-182/183 targets DCC and negatively regulates DCC: (a) Venn diagram of the predicted target genes of miR-182 and miR-183 by the RNA22 database. The blue circle represents the prediction result of miR-182, the red circle represents the prediction result of miR-183, and the middle part represents the intersection of the two sets of data. (b–d) GO function analysis of miRNA target gene prediction results; (b–d) enrichment results of three GO functional groups: biological process (BP), cellular component (CC), and molecular function (MF). The abscissa indicates the generation, and the ordinate indicates the name of entry identifiers. The circle size and color in the figure indicate the number of genes in the entry identifier and the value, respectively. (e) KEGG enrichment analysis of metabolic pathway in relation to miRNA target gene prediction results; (f) Western blot analysis of DCC protein in the hippocampal tissues of SZ () and normal () rats; (g) the binding site between miR-182/183 and DCC predicted by the bioinformatics website https://cm.jefferson.edu/rna22/; (h) binding of miR-182/183 to DCC determined by dual-luciferase reporter assay; (i) Western blot analysis of DCC protein in the hippocampal neurons treated with miR-182/183 mimic or AntimiR-182/183. The data in the figure were measurement data, expressed as the , and analyzed by an independent sample -test. vs. the normal or miR-182/183 mimic-NC group. ^#vs. the AntimiR control group. The cell experiments were repeated three times independently.

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