MicroRNA-1224-5p Aggravates Sepsis-Related Acute Lung Injury in Mice

Figure 1

The miR-1224-5p antagomir attenuates LPS-induced ALI in mice. (a) Mice were intratracheally injected with LPS (5 mg/kg), and the levels of miR-1224-5p in lungs were measured at indicating times. (b) Mice were intratracheally injected with indicating doses of LPS for 12 h, and the levels of miR-1224-5p in lungs were measured. (c) Mice were intravenously injected with the miR-1224-5p antagomir (80 mg/kg/day) or AntagNC for 3 consecutive days and then exposed to LPS (5 mg/kg) for 12 h, and the levels of miR-1224-5p in lungs were measured. (d)–(h) Pulmonary function was detected. (i, j) Arterial blood gas analysis of PaO₂, PaCO₂, and HCO₃^-. (k) Lung wet to dry ratio. (l) LDH activities in lungs. (m) Total protein concentrations in BALF. (n) Mice were intravenously injected with the miR-1224-5p antagomir (80 mg/kg/day) or antagNC for 3 consecutive days and then exposed to a lethal dose of LPS (25 mg/kg), and the survival rate was calculated every 12 h. Data represent the ( per group). versus the matched group.

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