MicroRNA-1224-5p Aggravates Sepsis-Related Acute Lung Injury in Mice

Figure 5

The miR-1224-5p antagomir alleviates oxidative stress and inflammation in LPS-stimulated macrophages. (a) Macrophages were pretreated with the miR-1224-5p antagomir (50 nmol/L) for 24 h, cultured in fresh medium for an additional 24 h, and then stimulated with or without LPS (100 ng/mL) for 6 h. The levels of miR-1224-5p were detected. (b) ROS levels in macrophages were measured by a DCFH-DA probe. (c) MDA generations in macrophages. (d) Total SOD activities and GSH content in macrophages. (e) NF-κB activities in macrophages. (f) The levels of IL-6 and TNF-α in the culture of macrophages. (g) Relative caspase-1 activities in macrophages. (h, i) Protein levels of TXNIP, NLRP3, and caspase-1 p10 in macrophages. (j) The levels of IL-1β and IL-18 in the culture of macrophages. Data represent the ( per group). versus the matched group.

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