Xanthohumol Protects the Rat Myocardium against Ischemia/Reperfusion Injury-Induced Ferroptosis

Figure 2

XN reduces ROS and iron accumulation in H9c2 cardiomyoblasts. (a) Treatment with Fe-SP (0.5 μM) or RSL3 (0.1 μM) for 16 h increased the intracellular ROS, and this effect was abolished by cotreatment with XN (10 or 20 μM). Left panel shows the DCFDA intensity, a fluorescence indicator of intracellular ROS. Right panel shows a graph of quantitation of these data (n =5). (b) The ROS image was detected by confocal microscopy using CM-H₂DCFDA. XN (10 or 20 μM) or a ferrous iron chelator, phen (50 μM) reduced the intracellular ROS in the Fe-SP- or RSL3-treated H9c2 cardiomyoblasts. Hoechst 33342 was used to identify the cell nuclei. . (c) Fe-SP, but not RSL3, increased the intracellular iron, and this effect was abolished by cotreatment with XN (20 μM) or phen (50 μM). Upper panel shows the PGSK intensity, a fluorescence indicator of intracellular iron. Bottom panel shows a graph of quantitation of these data (). All data represent . , versus the control group; ^#, versus the Fe-SP; ^$, versus the RSL3. Con: control; phen: 1, 10-phenanthroline; Hoe: Hoechst 33342.

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