Xanthohumol Protects the Rat Myocardium against Ischemia/Reperfusion Injury-Induced Ferroptosis

Figure 5

XN prevents the ischemia-reperfusion-induced ferroptosis in ex vivo hearts. (a) XN (5 μM) significantly attenuated the I/R-induced increase of myocardial infarct size (). XN (10 μM) significantly decreased the levels of Ptgs2 (b) and Acsl4 (c) mRNA in myocardial-treated with I/R, measured using quantitative PCR (). (d) Treatment with XN significantly inhibited the I/R-increased the level of ACSL4 protein enhancement (). (e) Treatment with XN increased the level of GPX4 protein as compared with the I/R-treated group (). (f) Treatment with XN did not significantly affect the I/R-reduced the level of FTH protein (). (g) Treatment with XN significantly inhibited the I/R-increased the level of NRF2 protein (). All data represent . , versus the control group; ^#, versus the I/R. Con: control; I/R: ischemia-reperfusion.

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