Research Article
SEMA6D, Negatively Regulated by miR-7, Contributes to C28/I2 chondrocyte’s Catabolic and Anabolic Activities via p38 Signaling Pathway
Figure 6
SEMA6D reversed the catabolism induced by FN-f. (a) The transfection effect of si-SEMA6D and pcDNA3.1-SEMA6D was verified by qRT-PCR. (b–c) Down-regulation of SEMA6D resulted in an overexpression of MMP-2 and MMP-13 both in normal chondrocytes and OP1-treated chondrocytes. (d) Western blotting was performed to measure the expression of MMP-2, MMP-13, and SEMA6D. , , , and indicates no significant difference compared with the control group.
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