Adaptation of the Oxygen Sensing System during Lung Development

Figure 1

Expression patterns of HIF1α protein and of bona fide HIF-regulated genes (HRGs) in the developing mouse lung. Densitometric (a) and representative (b) HIF1α protein stabilization was detected by immunoblotting to show the hypoxic state of the lung during intrauterine development and its destabilization at birth. β-Actin served as a loading control, mice per time point. (c–f) Absolute expression levels of bona fide HRGs were determined by qPCR ( mice per time point). One time point is represented by at least three different litters. Developmental stage is indicated by postconception days (E) or postnatal days (P) (for easier reference: (mlcs), , and ). Black bars represent the medians; the dashed line at 32 mlcs represents a physiological relevance threshold. The intrauterine period is indicated by the gray background color. Change points detected by the algorithms Sequen (#) or McDermott are indicated.

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