Adaptation of the Oxygen Sensing System during Lung Development

Figure 6

RNAscope in situ hybridization of the Hifs during different stages of lung development. (a) Developmental stage is indicated by postconception days (E) or postnatal days (P). (b) Singleplex fluorescent staining of mouse lung sections at E16 (pseudoglandular phase: I, IV, and VII), P3 (saccular phase: II, V, and VIII), and P7 (alveolar phase: III, VI, and IX) in red (Opal 650) and DAPI in blue. The probe for Hif3a detects both Hif3a and Nepas, but not Ipas. Scale bars indicate 50 μm. The intrauterine period is indicated by the gray background color.