Increased Expression of Prolyl Endopeptidase Induced by Oxidative Stress in Nucleus Pulposus Cells Aggravates Intervertebral Disc Degeneration

<div>PREP knockout protects against puncture-induced IVDD. (a, b) HE and Safranine O/fast green staining showed the severity of IVDD changes at 6 weeks after puncture. Scale bar, 200 <i>μ</i>m. (c) Immunohistochemical staining of MMP-3 in mouse intervertebral disc samples. Scale bar, 200 <i>μ</i>m. (d) X-ray examination of mouse caudal vertebrae. (e) Schematic diagram of disc height index (DHI). <span class="nowrap"><svg height="11.5564pt" id="M32" style="vertical-align:-2.26807pt" version="1.1" viewbox="-0.0498162 -9.28833 229.718 11.5564" width="229.718pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M43 650V622C120 616 128 612 128 526V124C128 39 120 33 34 27V0H270C392 0 492 25 567 83C643 141 690 230 690 350C690 444 655 517 605 565C543 625 450 650 323 650H43ZM213 547C213 587 217 598 226 604C236 612 262 617 304 617C371 617 429 604 474 576C554 529 592 439 592 336C592 176 505 36 319 36C246 36 213 55 213 131V547Z"></path></g><g transform="matrix(.013,0,0,-0.013,9.438,0)"><path d="M729 650H468V622C546 615 554 610 554 527V365H213V527C213 610 221 616 297 622V650H38V622C120 616 128 611 128 527V123C128 39 120 34 40 28V0H303V28C221 34 213 40 213 123V322H554V123C554 39 546 34 460 28V0H728V28C647 34 639 39 639 123V527C639 610 647 615 729 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Oxidative Medicine and Cellular Longevity

fig6

Figure 6

Figure 6: Increased Expression of Prolyl Endopeptidase Induced by Oxidative Stress in Nucleus Pulposus Cells Aggravates Intervertebral Disc Degeneration 

Figure 6 | Increased Expression of Prolyl Endopeptidase Induced by Oxidative Stress in Nucleus Pulposus Cells Aggravates Intervertebral Disc Degeneration 