Dapagliflozin Ameliorates Renal Tubular Ferroptosis in Diabetes via SLC40A1 Stabilization

Figure 4

Dapagliflozin ameliorates ferroptosis in HK-2 cell injury models in vitro. (a) The cell viability assay revealed a significant dose-dependent increase cell death in erastin-stimulated cells as compared to control cells; CCK-8 assays with different stimulating glucose (b) or PA (c) concentrations in HK-2 cells. (d) Dapagliflozin inhibited HG- or PA-induced cell death in HK-2 cells, and this effect was reversed by erastin; (e) iron ion, (f) GSH, and (g) MDA contents in these groups. (h) Quantification of the average band densities calculated from different western blots and the protein levels of GPX4 and SLC7A11 in HK-2 cells in these groups. (i) SGLT2-siRNA was validated by western blot and the expression of SLC7A11 and GPX4 in those groups. Values are the . independent experiments. vs. NC; ^# vs. HG or erastin 1 μM group; ^¥ vs. PA; ^& vs. HG+DAPA; ^@ vs. PA+DAPA.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)