Four-Octyl Itaconate Attenuates UVB-Induced Melanocytes and Keratinocytes Apoptosis by Nrf2 Activation-Dependent ROS Inhibition

Figure 1

4-OI inhibited UVB-induced cytotoxicity and apoptosis in a concentration-dependent manner in HaCaT and PIG1 cells. (a) HaCaT and PIG1 cells were exposed to different doses of UVB (0, 120, 240, and 300 mJ/cm²) and investigated 24 h postirradiation. (b) HaCaT and PIG1 cells were treated with different concentrations of 4-OI (0, 1, 10, 100, and 500 μM) for 24 h. (c) HaCaT and PIG1 cells were pretreated with 4-OI (50 and 100 μM) for 24 h, then exposed to 300 mJ/cm² UVB, and investigated 24 h postirradiation. The cell viability was determined by CCK-8 cell viability assay. Cellular viability rate and statistical significance were determined with respect to the 100% viability of untreated control cells. HaCaT and PIG1 cells were exposed to UVB (300 mJ/cm²) with or without pretreatment with 4-OI (50 and 100 μM) and investigated 24 h postirradiation. (d) Morphological changes. Magnification: ×20. (e) Cell apoptosis by flow cytometry. Data were presented as () of one representative experiment of three. , , and versus the control group; ^#, ^##, and ^### versus the UVB group.

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