Four-Octyl Itaconate Attenuates UVB-Induced Melanocytes and Keratinocytes Apoptosis by Nrf2 Activation-Dependent ROS Inhibition

Figure 2

UVB induced cell apoptosis through ROS production. HaCaT and PIG1 cells were exposed to UVB (300 mJ/cm²) with or without 1 h pretreatment with NAC (5 mM). Twenty-four hours after stimulation, cells were incubated with DCFH-DA, and the level of ROS production was detected by (a) fluorescence microscopy ( μm) and (b) flow cytometric analysis. (c) Apoptosis of HaCaT and PIG1 cells was investigated 24 h postirradiation by flow cytometry. Data are expressed as () of one representative experiment of three. versus the control group; ^### versus the UVB group.

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