Research Article
Four-Octyl Itaconate Attenuates UVB-Induced Melanocytes and Keratinocytes Apoptosis by Nrf2 Activation-Dependent ROS Inhibition
Figure 4
4-OI rescued UVB-induced cell apoptosis via Nrf2/HO-1 activation-dependent ROS inhibition. (a) Western blotting analysis for the effect of si-Nrf2 transfection in HaCaT and PIG1 cells. si-control or si-Nrf2-transfected cells were pretreated with 4-OI (100 μM) for 24 h prior to UVB exposure (300 mJ/cm2); then, the ROS levels (b) and apoptosis (c) were determined by flow cytometry. Results are expressed as () of one representative experiment of at least three. , , and versus the control group.
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