LncRNA-XIST Promotes Proliferation and Migration in ox-LDL Stimulated Vascular Smooth Muscle Cells through miR-539-5p/SPP1 Axis

Figure 5

Effects of miR-539-5p on proliferation, migration, and invasion of ox-LDL stimulated VSMCs. (a) RT-qPCR was used to detect the efficiency of overexpression miR-539-5p in VSMCs. (b) The cell viability of VSMCs after overexpression of miR-539-5p was detected by CCK-8 assay in NC mimic group vs. miR-539-5p mimic group. (c) The expression levels of Ki67 and PCNA protein. (d)–(f) Wound-healing assay () and transwell assay () showed the effects on invasion and migration of VSMCs. The bar graph showed the percentage of cells in each group. (g) The expression of MMP-2 and MMP-9 protein detected by western blot analyses. , , ^#, ^##, ^###.

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