Forsythiaside A Regulates Activation of Hepatic Stellate Cells by Inhibiting NOX4-Dependent ROS

Figure 2

FA inhibited the proliferation and migration of LX2 cells. (A) The chemical formula of FA (https://pubchem.ncbi.nlm.nih.gov/). (B) Effects of different concentrations of FA (0-400 μmol/L) on LX2 cell viability. p <0.001 FA vs control (C) The effects of FA and TGF-β1 treatment on cell viability. (D) The morphology of LX2 cells was observed under an inverted microscope (100X). (E) Cell migration was observed at 0 h, 12 h, 18 h, 24 h after FA treatment (100X). Data from the four independent trials. (F) Relative migration rate was used as a quantitative measure of cell migration. Data were represented by mean ± S.D. p <0.001 TGF-β1 vs control; p <0.05 TGF-β1 vs control. ^###p <0.001 TGF-β1 + FA vs TGF-β1;^##p <0.01 TGF-β1 + FA vs TGF-β1; ^#p <0.05 TGF-β1 + FA vs TGF-β1.

(a)

(b)

(c)

(d)

(e)

(f)