Polydatin Attenuates Cisplatin-Induced Acute Kidney Injury by Inhibiting Ferroptosis

Figure 2

Effects of PD on inhibiting ferroptosis in Cis-AKI mice. (a) The ultrastructure of the renal tissue was seized by TEM at 48 h after cisplatin injection (12000x magnification). (b–d) The lipid peroxidation markers, immunohistochemistry for 4HNE (400x magnification), and determination of MDA and LPO were performed at 48 h after cisplatin injection. (e, f) The level of GSH and the activity of GPx4 were tested in kidneys at 48 h after cisplatin injection. (g) Levels of chelatable iron in kidneys were determined by EPR detection of ferrioxamine. , vs. control; ^#, ^## vs. cisplatin; ^$, ^$$ vs. Cis+PD ( to 7). Cis: cisplatin; PD: polydatin; Fer-1: ferrostatin-1; TEM: transmission electron microscopy; 4HNE: 4-hydroxynonenal; MDA: malondialdehyde; LPO: lipid hydroperoxide; GSH: glutathione; GPx4: glutathione peroxidase-4; LIP: labile iron pool.

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