Apolipoprotein (a)/Lipoprotein(a)-Induced Oxidative-Inflammatory α7-nAChR/p38 MAPK/IL-6/RhoA-GTP Signaling Axis and M1 Macrophage Polarization Modulate Inflammation-Associated Development of Coronary Artery Spasm

Figure 3

Lp(a) preferentially induces patient monocyte-derived macrophage M1 polarization. Dots and line plot showing the expression profile of (a) CHRNA7, (b) CD163, (c) CD206, or (d) CD80 in the macrophages, resting monocytes, stem cells, stimulated monocytes, and T cells of patients with CAD using the GSE9820/GPL6255/GDS3690 dataset, . Flow cytometry cell count polygons (upper panel) and histograms (lower panel) depicting the effect of treatment with 500 nM LDL or Lp(a) on the (e) CD80 median fluorescence intensity or (f) CD206 median fluorescence intensity of CAS monocyte-derived macrophages. Histogram colors are green for control antibody and purple for target antibody. The macrophages were exposed to either Lp(a) or LDL for 24 hours. LDL: low-density lipoprotein; PE: phycoerythrin. , , and .

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