Research Article

A Mixture of Ginkgo biloba L. Leaf and Hericium erinaceus (Bull.) Pers. Fruit Extract Attenuates Scopolamine-Induced Memory Impairments in Mice

Figure 3

Effect of the mixture (GH) of Ginkgo biloba L. leaf (GL) and Hericium erinaceus (Bull.) Pers. (HE) fruit extracts on scopolamine- (Sco-) induced ROS production, mitochondrial dysfunction, and apoptosis pathways in human neuroblastoma (SH-SY5Y) cells. (a) ROS production was measured using the DCF-DA staining assay. (b) Mitochondrial membrane potential was measured for evaluating mitochondrial dysfunction using the JC-10 assay kit. (c, d) Western blotting assay of Bax, Bcl-2, cleaved- (C-) caspase-3, and total- (T-) caspase-3 was performed, with GAPDH as a loading control. Quantification was calculated using densitometric analysis using Bio-Rad Quantity software. Data represent the . ## and ### vs. control group. vs. Sco-treated group.