Simvastatin Reduces NETosis to Attenuate Severe Asthma by Inhibiting PAD4 Expression

Figure 1

Simvastatin treatment ameliorates features of TH2-mediated and TH17-mediated asthma in mice. (a) Measurement of dynamic airway resistance. (b–d) Total cell counts, eosinophil counts, and neutrophil counts (10⁵ cells/ml) in the BALF. (e–h) Levels of IL-1β, IFN-γ, IL-4, and IL-17 in the BALF. (i, j) Representative hematoxylin and eosin (H&E) staining and inflammation score of lung sections of mice. (k, l) Representative PAS staining of lung sections and quantification of PAS-stained epithelial cells per bronchi showing airway mucus production in mice. (m–r) Flow cytometric analysis of percentages of TH2, TH17, and Treg lymphocytes in the lung.