Long Noncoding RNA LINC00578 Inhibits Ferroptosis in Pancreatic Cancer via Regulating SLC7A11 Ubiquitination

Figure 2

LINC00578 promotes pancreatic cancer cell proliferation and invasion in vitro and tumor growth in vivo. (a) CCK-8 assays were applied to detect cell viability at the indicated time points in PATU8988 cells transfected with LINC00578 (OE-LINC00578) versus empty vector-transfected PATU8988 cells (Vector) and LINC00578 knockdown PL45 cells (Sh-LINC00578) versus empty vector knockdown PL45 cells (Sh-NC). (b) Colony formation assays were applied to detect cell proliferation in PATU8988 cells (OE-LINC00578 group versus vector group) and PL45 cells (Sh-LINC00578 group versus Sh-NC group). (c) Wound-healing assay indicating LINC00578-induced increased migration in PATU8988 and PL45 cells. (d) Tumor size, volume, and weight at day 30 after subcutaneous injection of LINC00578-overexpressing PATU8988 cells () compared with the vector group (). (e) A nucleocytoplasmic separation assay was used to determine the intracellular location of LINC00578 in PATU8988 cells and PL45 cells. ; ; .

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