Long Noncoding RNA LINC00578 Inhibits Ferroptosis in Pancreatic Cancer via Regulating SLC7A11 Ubiquitination

Figure 3

LINC00578 acts as a ferroptosis repressor in pancreatic cancer. (a) Statistical distribution of differentially expressed proteins in GO secondary classification. Blue represents cellular components. Yellow represents molecular function. Green represents biological processes. (b) Bubble map showing the distribution of differentially expressed proteins (Sh-LINC00578 group versus Sh-NC group, selected by label-free proteomics) enriched in the KEGG pathway. (c) Quantitative analysis of intracellular Fe²⁺. (d) Quantitative analysis of intracellular malondialdehyde (MDA). (e) OE-LINC00578, vector, Sh-LINC00578, and Sh-NC cells were stained with DCFH-DA to measure intracellular ROS levels via flow cytometry. (f) Quantitative analysis of ROS generation. (g) OE-LINC00578, vector, Sh-LINC00578, and Sh-NC cells were stained with JC-1 to measure MMP levels via flow cytometry. Green represents the depolarized state of cells. Red represents the normal state of cells. (h) Quantitative analysis of MMP. Red/green ratio showing the effect of LINC00578 on inhibiting MMP depolarization. ; ; ; .

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