Long Noncoding RNA LINC00578 Inhibits Ferroptosis in Pancreatic Cancer via Regulating SLC7A11 Ubiquitination

Figure 4

LINC00578 negatively modulates ferroptosis by targeting SLC7A11. (a) Observation of cell number changes in the OE-LINC00578 group versus the vector group under the induction of DMSO, erastin, and erastin+Fer-1. (b) CCK-8 assay showing the relative cell viability in the OE-LINC00578 group versus the vector group after induction with DMSO, erastin, and erastin+Fer-1. (c) Western blot results showing the levels of ferroptosis-related proteins (SLC7A11, P53, GPX4, Survivin, and GPX4) in pancreatic cancer cells. (d) Quantitative analysis of the levels of ferroptosis-related proteins. (e) Western blotting to measure the protein level of SLC7A11 in PATU8988 cells with or without erastin induction. (f) Quantitative analysis of the protein level of SLC7A11. (g) SLC7A11 silencing inhibited LINC00578-induced ferroptosis-related protein (SLC7A11, P53, GPX4, Survivin, and GPX4) expression. (h) Quantitative analysis of the protein level of LINC00578-induced ferroptosis-related proteins (SLC7A11, P53, GPX4, Survivin, and GPX4) reversed by SLC7A11 silencing. ; ; and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)