Sulforaphane Delays Intervertebral Disc Degeneration by Alleviating Endoplasmic Reticulum Stress in Nucleus Pulposus Cells via Activating Nrf-2/HO-1

Figure 1

ERS level during IVDD in human NP tissues. (a) Magnetic resonance images of intervertebral discs of five different degenerative grades. (b–d) WB was used to detect the expression of ERS-related proteins in IVD of different degenerative grades, and the relative quantitative data (c, d) was calculated accordingly. vs. any Pfirrmann grading. (e) GRP78 mRNA level was measured by qRT-PCR in normal and degenerative NP tissues. (f) Correlation analysis between GRP78 mRNA level and Pfirrmann grading. . (g) CHOP mRNA level was measured by qRT-PCR in normal and degenerative NP tissues. (h) Correlation analysis between CHOP mRNA level and Pfirrmann grading. . (i–l) Representative images of caspase-3 (i) and caspase-12 (k) expression was detected by immunofluorescence analysis and the relative fluorescence intensity was calculated in normal and degenerative NP tissues. vs. NC group. Scale  μm. (Error bars showed ; ).

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