Sulforaphane Delays Intervertebral Disc Degeneration by Alleviating Endoplasmic Reticulum Stress in Nucleus Pulposus Cells via Activating Nrf-2/HO-1

Figure 5

SFN promoted Nrf-2 translocation into the nucleus. (a, b) Nrf-2 expression in the cytoplasm was detected by WB. (c, d) Nrf-2 expression in the nucleus was detected by WB. (e, f) Nrf-2 fluorescence staining showed its distribution in NPCs. The nucleus was dyed blue, Nrf-2 was dyed red, and Nrf-2 in the nucleus was purple. Scale  μm. (g) WB was used to detect the expression of HO-1 protein. (h) HO-1 mRNA level was measured by qRT-PCR. (Error bars showed ; ; , , , vs. control group; ^#, ^##, ^###, vs. AGEs group).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)