Sulforaphane Delays Intervertebral Disc Degeneration by Alleviating Endoplasmic Reticulum Stress in Nucleus Pulposus Cells via Activating Nrf-2/HO-1

Figure 6

SFN delayed IVDD in vivo. (a) The tails of rats were examined by magnetic resonance at 0, 4, and 8 weeks, respectively. (b) The rat IVD were scored according to the magnetic resonance images. (c) Representative images of H&E staining of IVD. Scale bar  μm; scale bar  μm. (d) Representative images of Safranin-O/fast green staining of IVD. Scale bar  μm; scale bar  μm. (e) Histological score of IVD. (f, g) Representative images of TUNEL staining of IVD and quantification analysis were performed. Scale  μm. (h, i) Immunohistochemistry was used to detect ERS-related proteins and Nrf-2/HO-1 in the NP, and quantification analysis were performed. Scale  μm. (Error bars showed ; ; , vs. PBS group; ^###, vs. AGEs group).

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