Cryptotanshinone Attenuated Pathological Cardiac Remodeling In Vivo and In Vitro Experiments

Figure 3

CTS treatment attenuated mouse cardiac fibrosis. (a) PSR staining to detected interstitial and perivascular fibrosis in the left ventricle (); (b) calculation of the PSR-positive area in the left ventricle according to PSR staining (); (c) representative Western blots for FN, α-SMA, and GAPDH (); (d) relative quantification of FN/GAPDH and α-SMA/GAPDH (); RT-PCR examined the mRNA expression of (e) Tgfb, (f) CCN2, (g) COL1A1, and (h) COL3A1 in indicated groups (). GAPDH was used for loading control. The protein and mRNA expression was normalized to GAPDH at its protein or mRNA expression, respectively, before the relative quantification. Data presented as the One-way ANOVA followed by Tukey’s post hoc test was used for examining the significance difference among different groups. Compared with the sham group (); ^#compared with the AB+VEH group ().

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