Baicalin Inhibits Airway Smooth Muscle Cells Proliferation through the RAS Signaling Pathway in Murine Asthmatic Airway Remodeling Model

Figure 4

Effects of A-RAF shRNA on RAS signaling pathway and cell proliferation. (a) The mRNA expression of A-RAF was measured by qPCR in HEK293T cell after A-RAF ablation. (b) The protein expression of A-RAF was measured by western blot in HEK293T cell after A-RAF ablation. (c) The expression of differential proteins in key branching pathway of HEK293T cell was detected by western blot after A-RAF ablation. The relative density of each protein was calculated. (d) The proliferation of HEK293T cell was detected by the EdU (DAB) test. (e) The proliferation of HEK293T cell was detected by MTT assay. All data were shown as . All the data are representative of from each group. versus shNC.

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