Dietary High-Fat Promotes Cognitive Impairment by Suppressing Mitophagy

Figure 3

Enhanced mitophagy ameliorates PA-induced cellular senescence. (a) N2a cell treatment protocol. (b) Compared with the control group and PA + Kaem group, PA-exposed cells showed more senescent cell morphology and stained positive for SA-β-Gal. . (c) Red fluorescence represents the aggregated forms of JC-1 mitochondria, suggesting an intact mitochondrial membrane potential. The green fluorescence represents the monomeric form of JC-1, suggesting a lowered membrane potential. (d) The relative proportion of cells with red and green fluorescence was used to measure mitochondrial depolarization (, one-way ANOVA). . Data are shown as ; , . Abbreviations: N2a: Neuro-2a; Kaem: kaemferol; CON: control; PA: palmitic acid.

(a)

(b)

(c)

(d)