Research Article
Overexpression of PRDX2 in Adipose-Derived Mesenchymal Stem Cells Enhances the Therapeutic Effect in a Neurogenic Erectile Dysfunction Rat Model by Inhibiting Ferroptosis
Figure 2
PRDX2 exerts antiapoptotic and the antioxidant stress and promotes proliferation in ADSCs exposed to H2O2 in vitro. (a, b) Apoptosis in ADSCs and PRDX2-overexpressing ADSCs cultured under H2O2 stimulation was analyzed using Annexin V-APC/7-AAD staining and flow cytometry (). (c, d) Intracellular ROS levels in ADSCs and PRDX2-overexpressing ADSCs cultured under H2O2 stimulation were evaluated using DHE-ROS and flow cytometry (). (e, f) Intracellular ROS levels in ADSCs and PRDX2-overexpressing ADSCs cultured under H2O2 stimulation were determined using DHE-ROS and fluorescence microscope (). (g) Effect of PRDX2 overexpression on ADSC viability by CCK-8 assay (). ADSC-P, ADSCs transfected with PRDX2. , , , and . MFI: mean fluorescence intensity.
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