Overexpression of PRDX2 in Adipose-Derived Mesenchymal Stem Cells Enhances the Therapeutic Effect in a Neurogenic Erectile Dysfunction Rat Model by Inhibiting Ferroptosis

Figure 3

PRDX2 overexpression in ADSCs alleviates H₂O₂-induced CCSMC apoptosis and oxidative stress. (a) The diagram of the coculture system of ADSCs and CCSMCs. (b, c) Morphology of CCSMCs in passage 0 and passage 3. (d) Immunofluorescence of calponin 1 to identify CCSMCs. (e) The proliferation of CCSMCs cocultured with vehicle-ADSCs or PRDX2-ADSCs under H₂O₂ stimulation was analyzed by CCK-8 assay (). (f–i) Intracellular ROS, superoxide anion, and mitochondrial superoxide levels of CCSMCs after coculture as in (e) were determined using DCFH-DA, DHE, MitoSOX, and fluorescence microscope (). (j, k) Intracellular ROS levels of CCSMCs after coculture as in D using DCFH-DA and flow cytometry (). NC, CCSMCs without any treatment. Control, CCSMCs treated with H₂O₂ treatment. Vehicle, CCSMCs treated with H₂O₂ treatment and cocultured with ADSCs transfected with empty vector. ADSC-P, CCSMCs treated with H₂O₂ treatment and cocultured with ADSCs transfected with PRDX2. , , , and . MFI: mean fluorescence intensity.

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