Overexpression of PRDX2 in Adipose-Derived Mesenchymal Stem Cells Enhances the Therapeutic Effect in a Neurogenic Erectile Dysfunction Rat Model by Inhibiting Ferroptosis

Figure 7

PRDX2-ADSC treatment attenuated ferroptosis in RSL3-exposed CCSMCs via GPX4/ACSL4 axis. (a) The proliferation of CCSMCs cocultured with vehicle-ADSCs or PRDX2-overexpressing ADSCs under RSL3 stimulation was analyzed by CCK-8 assay (). (b–e) The levels of total iron content, MDA, SOD, and GSH in CCSMCs of each group were measured by commercially assay kits (). (h–j) Representative western blotting results and quantification of GPX4 and ACSL4 in CCSMCs of each group (). (f, g, k, l) Representative fluorescence images and quantification of GPX4 and ACSL4 in CCSMCs of each group (). NC, CCSMCs without any treatment. Control, CCSMCs treated with RSL3 treatment. Vehicle, CCSMCs treated with RSL3 treatment and cocultured with ADSCs transfected with empty vector. ADSC-P, CCSMCs treated with RSL3 treatment and cocultured with ADSCs transfected with PRDX2. , , , and . MFI: mean fluorescence intensity.

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