α-Synuclein Attenuates Maneb Neurotoxicity through the Modulation of Redox-Sensitive Transcription Factors

Figure 2

α-Synuclein expression and phosphorylation are affected to a different extent by MB in cells with endogenous and overexpressed levels of the protein. (a) α-Synuclein expression levels were determined in wt α-syn and control cells (IMR-32 cells stably transfected with pcDNA-human wild-type α-synuclein plasmid or the empty vector, respectively) by Western blot in total cellular lysates. β-Actin was used as loading control. A representative blot of three different experiments is shown. Band quantification was performed with ImageJ. Results are expressed as arbitrary units and represent (). (b, c) Analysis of α-synuclein expression and phosphorylation in control and wt α-syn cells exposed to MB (24 μM) or vehicle for 48 h was performed by immunocytochemistry studies. Hoechst was used as nuclear marker. Fluorescence intensity quantification was performed with ImageJ, and results are expressed as arbitrary units. Scale bars: 20 μm. (a–c) and for each condition with respect to the corresponding control; one-way ANOVA and Tukey’s post hoc test.

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