α-Synuclein Attenuates Maneb Neurotoxicity through the Modulation of Redox-Sensitive Transcription Factors

Figure 4

Effect of α-synuclein overexpression on the state of transcription factors involved in cellular responses to oxidative stress. (a, b) Immunocytochemistry was used to analyze NRF2 and FOXO3a cellular localization in cells with endogenous and overexpressed levels of α-synuclein (control and wt α-syn cells, respectively). Hoechst was used as nuclear marker. Fluorescence intensity was quantified using ImageJ. Pictures represent at least three different experiments. Scale bars: 20 μm. (c) Western blot analysis of FOXO3a levels was performed in nuclear (N) and cytosolic (C) fractions. hnRNP was used as nuclear marker and β-actin as loading control. Representative blots of three different experiments are shown. Band quantification was performed with ImageJ. (a–c) Results are expressed as percentage of the control or arbitrary units and represent (). and for each condition with respect to the control; one-way ANOVA and Tukey’s post hoc test.

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